Expression vectors able to elicit improved immune response and methods of using same

Vecteurs d'expression permettant de provoquer une reponse immunitaire et procedes d'utilisation desdits vecteurs

Abstract

L'invention concerne des acides nucléiques (tels que des plasmides d'immunisation d'ADN), codant des protéines hybrides contenant une séquence d'acides aminés déstabilisante attachée à une séquence d'acides aminés recherchée, dans lesquels le caractère immunogène de la séquence d'acides aminés recherchée est accrue par la présence de la séquence d'acides aminés déstabilisante. L'invention concerne également des acides nucléiques codant des protéines hybrides sécrétées, telles que celles contenant des chimiokines ou des cytokines, et une séquence d'acides aminés attachée recherchée, le caractère immunogène de la séquence d'acides aminés recherchée étant accru dans lesdits acides nucléiques du fait que cette dernière est attachée à la séquence de sécrétion. L'invention concerne également des procédés visant à augmenter le caractère immunogène des protéines codées destinées à être utilisées en tant que vaccins ou dans une thérapie génique.
The invention relates to nucleic acids (such as DNA immunization plasmids), encoding fusion proteins containing a destabilizing amino acid sequence attached to an amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased by the presence of the destabilizing amino acid sequence. The invention also relates to nucleic acids encoding secreted fusion proteins, such as those containing chemokines or cytokines, and an attached amino acid sequence of interest, in which the immunogenicity of the amino acid sequence of interest is increased as a result of being attached to the secretory sequence. The invention also relates methods of increasing the immunogenicity of the encoded proteins for use as vaccines or in gene therapy.

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