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Organic compounds

Composés organiques

Abstract

The present invention relates to a new process for the production of a polypeptide of interest, or of a precursor thereof, wherein the protein or the precursor are expressed in a host cell comprising a cell wall and at least one cell membrane, followed by extraction from the intact host cell by partial permeabilization of the cell membrane under non-denaturing conditions. During extraction the polypeptide of interest, or the precursor thereof, which is expressed in at least partially correctly folded form, passes through the cell wall and the cell membrane.

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    US-9187543-B2November 17, 2015Pfenex Inc.Method for producing soluble recombinant interferon protein without denaturing
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    WO-2011109556-A3January 12, 2012Pfenex Inc.Method for producing soluble recombinant interferon protein without denaturing