Analyse homogène à base de polarisation fluorescente pour détermination désoxynivalénol en grains

Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains

Polarisationsbasierter, homogener Fluoreszenz-Assay zur Bestimmung von Deoxynivalenol in Körnern

Abstract

The invention relates to a homogeneous assay for determining the deoxynivanlenol (DON) content in grains wherein the technique of fluorescence polarization is used. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.

Claims

Description

Topics

Download Full PDF Version (Non-Commercial Use)

Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
    DE-4013004-A1October 31, 1991Elvira ScheckliesELISA determn. of low mol. wt. pesticides, antibiotics or toxins - using enzyme labelled antibody reactant, providing lower detection limit

NO-Patent Citations (3)

    Title
    ADAMCZYK M ET AL: "SYNTHESIS OF CONJUGATES FOR A BARBITURATE SCREENING ASSAY", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 8, no. 3, 1 January 1997 (1997-01-01), pages 281 - 288, XP002085589, ISSN: 1043-1802
    LIGLER F S ET AL: "A HOMOGENEOUS IMMUNOASSAY FOR THE MYCOTOXIN T-2 UTILIZING LIPOSOMES MONOCLONAL ANTIBODIES AND COMPLEMENT", 1 January 1987, ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC. NEW YORK, PAGE(S) 369 - 375, ISSN: 0003-2697, XP001062106
    NIELSEN K ET AL: "A homogeneous fluorescence polarization assay for detection of antibody to Brucella abortus", 9 September 1996, JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, PAGE(S) 161 - 168, ISSN: 0022-1759, XP004021266

Cited By (0)

    Publication numberPublication dateAssigneeTitle